Composite

Part:BBa_K4790109:Design

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-10-11)


ASR2(node 98)/pET-22b(+)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1967
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
    Illegal NotI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 499
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 635
    Illegal PstI site found at 1091
    Illegal PstI site found at 1292
    Illegal NgoMIV site found at 143
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 1198
    Illegal NgoMIV site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The original sequence of pET-22b was downloaded from NCBI. Node 98 was constructed through Ancestral Sequence Reconstruction (ASR).

References